It differs from prokaryotes in the following respects:
(1) Prokaryotes have only one type of RNA polymerase for synthesis of mRNA, r-RNA and t-RNA while the eukaryotes have three different types of RNA polymerases, RNA polymerase I for ribosomal RNA, RNA polymerase II for heterogeneous nuclear RNA which ultimately is processed to mRNA and RNA polymerase III for transfer RNA.
(2) In prokaryotes, the cell is a single compartment and the translation of m-RNA starts even when it has not been fully transcribed. Thus the transcription and translation of the same mRNA go together. On the contrary, eukaryotic cells have developed isolated compartments like nucleus, chloroplast, mitochondria etc.
The synthesis of r-RNA is limited only to nucleolar region while the mRNA and t-RNA are synthesized in nucleoplasm. However, the translation of mRNA takes place outside the nucleus in the cytoplasm. Thus the transcription and translation are separated in space and time in eukaryotes.
(3) In prokaryotes the primary transcript mRNA (nascent RNA) is translated without any processing/modification while the primary transcript of mRNA (pre-mRNA) in eukaryotes has a number of intervening sequences which must be spliced to obtain a functional mRNA. Since the mRNA in eukaryotes is transported to the cytoplasm for translation, both the ends are modified to provide protection against nucleases.